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Analytical assessment of MALDI-TOF Imaging Mass Spectrometry on thin histological samples. An insight in proteome investigation
- Source :
-
Clinica Chimica Acta . Jul2005, Vol. 357 Issue 2, p210-218. 9p. - Publication Year :
- 2005
-
Abstract
- Abstract: Background: The development of MALDI Imaging Mass Spectrometry is a promising technique in the investigation of biological molecular repertoire. We have pursued an analytical assessment of this technique in its application to proteome analysis. Methods: A specific statistical method of analysis has been developed to enable data processing in the absence of internal standards, by defining similarity scores. Results: The investigated linear mode MALDI-TOF set-up allows to obtain data variations comprised within the 30% of variation when assaying tissues samples from the same animal, while the 60% of variation was highlighted in the inter-mice series assaying syngenic animals. Conclusions: This analytical assessment represents the first step of a process that should validate the utilisation of this technique in the clinical practice. [Copyright &y& Elsevier]
- Subjects :
- *PROTEOMICS
*MASS spectrometry
*MOLECULAR biology
*SPECTRUM analysis
Subjects
Details
- Language :
- English
- ISSN :
- 00098981
- Volume :
- 357
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Clinica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 18094413
- Full Text :
- https://doi.org/10.1016/j.cccn.2005.03.029