电针对紫杉醇诱导大鼠神经病理性疼痛的影响.

Objective To observe the effect of electroacupuncture (EA) on the expression of NKCC1, KCC2 and activation of microglia in spinal dorsal horn of paclitaxel (PTX)-induced neuropathic pain rats and its possible mechanism. Methods Male SD rats were randomly divided into the vehicle group (vehicle), the PTX group, the PTX + EA group and the PTX + sham EA group, with 12 rats in each group. The rat model of PTX-induced neuropathic pain was established by intraperitoneal injection of PTX. After modeling, EA was applied to "Zusanli" and "Yanglingquan" for 7 days in the PTX + EA group. Paw withdrawal threshold and paw withdrawal latency were tested at 2 days before and 1, 3, 5, 7, 14 and 21 days after PTX injection. Immunofluorescence and Western blot assay were used to detect expression levels of sodium-potassium-chloride cotransporter 1 (NKCC1), potassium-chloride cotransporters 2 (KCC2) and microglia markers - ionized calcium binding adapter molecule 1 (Iba1) in spinal dorsal horn. Results Compared with the vehicle group, mechanical and thermal hyperalgesia of both hind feet were found in the PTX group, and the expression of NKCC1 and the number of activated microglia in dorsal horn tissue of spinal cord were increased. Compared with the PTX group, mechanical and thermal hyperalgesia were significantly improved in the PTX+EA group at day 14 and 21, and the expression levels of NKCC1 and Iba1 in dorsal horn tissue of spinal cord were decreased. There was no significant difference in KCC2 expression between the four groups. Conclusion Electroacupuncture can effectively relieve paclitaxol-induced neuropathic pain, which may be related to the inhibition of NKCC1 expression and microglia activation in spinal dorsal horn of rats. [ABSTRACT FROM AUTHOR]