Novel analysis tool for the distance of gold dimers controlled by the DNA strand length on the DNA origami.

Metallic nanoparticle dimers have been used to enhance the excitation rate of single‐quantum emitters. The interparticle distance (<italic>d</italic>) of the dimers has a crucial influence on the signal enhancement. Therefore, precise control of <italic>d</italic> is desired for optimal performance. However, statistical analysis of <italic>d</italic> has been often restricted to a small number of dimers due to the lack of reliable automatic software tools.For this reason, we developed a novel analysis tool for automatic dimer analysis. Our approach combines particle detection by circle Hough transformation (CHT) with custom classification routines optimised for distinct types of particles. We applied our tool to scanning electron microscopy (SEM) images and achieved great agreement in dimer detection, reaching an agreement of around 97% between automatic analysis and manual inspection for more than 3000 metallic nanoparticle dimers on DNA origami controlled by a combination of multiple DNA strands.Our study revealed the effects of the strand length (<italic>L</italic>) on the distribution of <italic>d</italic>. Based on geometric consideration, we expected a strong correlation between <italic>L</italic> and the standard deviation (<italic>σ</italic>) of <italic>d</italic>. We could verify this correlation by characterising four dimer designs with different <italic>L</italic> while analysing more than 1000 dimers per specimen. [ABSTRACT FROM AUTHOR]