ACRYLONITRILE-INDUCED TOXOPATHOLOGICAL AND BIOCHEMICAL ALTERATIONS IN FEMALE ALBINO MICE.

Acrylonitrile (ACN) is an organic synthetic monomer that is widely used in food packaging and manufacturing. Our current study aimed to investigate toxic pathological effects and biochemical alterations in ACN-treated female mice. Seventy female mice were assigned into 3 groups and received oral distilled water, ACN (10.17 mg/kg b.w, 1/10 of LD50) twice a week and ACN (10.17 mg/kg b. w) three times a week, respectively. After 45, 90 and 120 days of treatment, blood samples and tissue specimens from the uteri were obtained for measurement of catalase (CAT) and malondialdehyde (MDA), and histopathological examination, respectively. It was found that ACN significantly reduced CAT levels in group 2 and 3 after 120 days of treatment compared to group 1. Moreover, CAT levels in group 3 were significantly decreased compared to that in group 2 after both times intervals, 45 and 90 days. ACN raised MDA concentration in group 3 after 120 days of treatment compared to groups 1 and 2. Histopathologically, ACN was seen to damage the uterus as it markedly caused congestion, hemorrhages, thrombosis, severe necrosis, and local and diffuse granulomatous inflammation. In conclusion, exposure of female mice to ACN induces pronounced hazardous toxic and pathological effects in the form of imbalance in the oxidantantioxidant harmony and marked histopathological changes. The study recommends avoiding overeating products containing ACN to keep proper health of the female genital system. [ABSTRACT FROM AUTHOR]