灰毡毛忍冬 SS 基因的克隆及表达分析.

[Objective] To clone the full-length sequence of the squalene synthase gene and perform bioinformatics and expression pattern analysis from Lonicera macranthoides. [Method] Total RNA was extracted from Lonicera macranthoides, and the full-length cDNA sequence of LmSS gene was cloned using RT-PCR and RACE techniques, bioinformatics analysis was conducted on the gene sequence using relevant software, the relative expression of the gene in stem, leaf, and different flower period was determined by using Real-time PCR. [Result] The open reading frame (ORF) of LmSS gene was 1 245 bp, encoding 414 amino acids. It belongs to a hydrophilic protein and is located in the cytoplasm. LmSS gene has a typical polyisoprene synthase active domain, which has high homology with other plant SS genes. The expression of LmSS gene is tissue-specific in different flowering stages and organs of Lonicera macranthoides. [Conclusion] This study successfully cloned the SS gene in Lonicera macranthoides, laying a foundation for further research on its function and providing a research basis for exploring the biosynthesis and regulatory mechanism of saponins in Lonicera macranthoides. [ABSTRACT FROM AUTHOR]