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Dental pulp stem cells promote malignant transformation of oral epithelial cells through mitochondrial transfer.

Dental pulp stem cells promote malignant transformation of oral epithelial cells through mitochondrial transfer.

Authors :
Shen, Peiqi
Ma, Zeyi
Xu, Xiaoqing
Li, Weiyu
Li, Yaoyin
Source :
Medical Molecular Morphology. Dec2024, Vol. 57 Issue 4, p306-319. 14p.
Publication Year :
2024

Abstract

Oral epithelial dysplasia includes a range of clinical oral mucosal diseases with potentially malignant traits. Dental pulp stem cells (DPSCs) are potential candidates for cell-based therapies targeting various diseases. However, the effect of DPSCs on the progression of oral mucosal precancerous lesions remains unclear. Animal experiments were conducted to assess the effect of human DPSCs (hDPSCs). We measured the proliferation, motility and mitochondrial respiratory function of the human dysplastic oral keratinocyte (DOK) cells cocultured with hDPSCs. Mitochondrial transfer experiments were performed to determine the role mitochondria from hDPSCs in the malignant transformation of DOK cells. hDPSCs injection accelerated carcinogenesis in 4NQO-induced oral epithelial dysplasia in mice. Coculture with hDPSCs increased the proliferation, migration, invasion and mitochondrial respiratory function of DOK cells. Mitochondria from hDPSCs could be transferred to DOK cells, and activated mTOR signaling pathway in DOK cells. Our study demonstrates that hDPSCs activate the mTOR signaling pathway through mitochondrial transfer, promoting the malignant transformation of oral precancerous epithelial lesions. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
18601480
Volume :
57
Issue :
4
Database :
Academic Search Index
Journal :
Medical Molecular Morphology
Publication Type :
Academic Journal
Accession number :
180733958
Full Text :
https://doi.org/10.1007/s00795-024-00403-1