Dynamic motion of mitochondria, plastids, and NAD(P)H zoning in Arabidopsis pollen tubes.

Pollen tubes consume a tremendous amount of energy and are the fastest-growing cells known in plants. Mitochondria are key organelles that supply energy and play important roles in modulating cellular redox homeostasis. Here, we found that endogenous NAD(P)H in Arabidopsis pollen tubes was spatially highly correlated with the distribution of mitochondria, both peaking in the subapex region. A weak association was also observed between the NAD(P)H levels and pollen plastids. Further studies using Class XI myosin mutants confirmed that altered mitochondrial distribution and trafficking concomitantly affected intracellular NAD(P)H zoning in pollen tubes. By targeting the NADPH- and NADH/NAD+-specific biosensors to the pollen tube cytosol of the myo11c1/myo11c2 double mutants, we showed that the growing pollen tubes in the double mutants possessed a lower level of cytosolic NADPH but a higher cytosolic NADH/NAD+ ratio than the WT. We also found that the knockout of Myo11C1 and Myo11C2 led to fragmented mitochondria with reduced motility. Therefore, altered cytosolic NAD(P)H levels may be secondary to changes in mitochondrial mobility, positioning, or morphology. Our results suggest that the spatial distribution and movement of mitochondria and plastids affect NAD(P)H zoning in Arabidopsis growing pollen tubes and that their movements depend on Class XI myosins. • Endogenous NAD(P)H of Arabidopsis pollen tube was spatially correlated with mitochondria and plastids. • Loss of Myo11C1 and Myo11C2 caused altered mitochondrial distribution and abnormal intracellular NAD(P)H zoning. • Mobility of pollen mitochondria and plastids was compromised in myo11c1/c2. • Myo11c1/c2 pollen tubes possessed reduced levels of cytosolic NADPH but higher cytosolic NADH/NAD+ ratios. [ABSTRACT FROM AUTHOR]