Integrated, high-throughput metabolomics approach for metabolite analysis of four sprout types.

In this study, we combined two distinct extraction and separation techniques with the aim of comprehensively collecting metabolite features in sprouts, particularly hydrophilic compounds. By synergistically analyzing the data using MS-DIAL and MetaboAnalystR, we obtained a greater number of annotated metabolites and explored differences in annotation across analytical tools. We found that this approach significantly increased the number of detected metabolite features and the final identification counts. Furthermore, we explored the functional component characteristics of four sprout types. This study provides data supporting the potential of sprouts as nutritious vegetables and functional food ingredients, emphasizing their value in the development of functional foods. • Explore the annotation differences between MS-DIAL and MetaboAnalystR for metabolomic data of sprouts • Integrate the metabolomics from methanol-C18 and water-HILIC to expand the coverage of features • The number of qualitative metabolites increased significantly with the comprehensive approach [ABSTRACT FROM AUTHOR]