基于报告基因法检测白细胞介素-11 生物学活性.

Interleukin-11 (IL-11) is a multifunctional cytokine that plays a crucial role in various biological processes, including the promotion of hematopoiesis, regulation of the immune system, and facilitation of tissue repair. These functions highlight its significant importance in both medical research and clinical therapy. Consequently, the precise detection and assessment of IL-11′s biological activity are essential. The currently employed cell proliferation inhibition assay is cumbersome, time-consuming, and susceptible to interference from Interleukin-6. Here we study the JAK-STAT signaling pathway, a key mechanism of IL-11 actions. We initially establish a stable monoclonal cell line that expresses a luciferase reporter gene responsive to IL-11. We subsequently optimized critical parameters, including the pre-dilution concentration and gradient of IL-11, cell inoculum size, and IL-11 incubation duration. Comprehensive validation was undertaken to evaluate accuracy, precision, specificity, stability, and concordance with pharmacopeial methods, ultimately leading to the establishment of a novel reporter gene-based approach for detecting the biological activity of IL-11. This innovative method significantly enhances the detection accuracy and sensitivity while reducing the testing time, thereby offering promising prospects for broad applications. [ABSTRACT FROM AUTHOR]