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Catalytic cleavage of an RNA substrate that bypasses the reorganization of its secondary structure during substrate recognition by a <italic>trans</italic>-acting VS ribozyme.

Authors :
Miyazaki, Yuki
Nakane, Ryu
Tanishi, Shogo
Matsumura, Shigeyoshi
Ikawa, Yoshiya
Source :
Nucleosides, Nucleotides & Nucleic Acids. Oct2024, p1-16. 16p. 5 Illustrations.
Publication Year :
2024

Abstract

AbstractVarkud satellite ribozyme (VS ribozyme) is a class of catalytic RNA with self-cleavage activity. The wild-type VS ribozyme has structural modularity with a relatively large catalytic module (H2–H6 elements) and a small substrate module (H1 element). The two modules can be dissected physically, and the substrate H1 RNA is recognized and then cleaved by the rest of the parent ribozyme serving as catalytic RNA. We characterized the catalytic properties of a bimolecular VS ribozyme developed and employed for an in-droplet evolution experiment of the VS ribozyme. We examined the effects of polyamines and several divalent metal ions. The results obtained in this study would be useful for the optimization of laboratory evolution of the VS ribozyme. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
15257770
Database :
Academic Search Index
Journal :
Nucleosides, Nucleotides & Nucleic Acids
Publication Type :
Academic Journal
Accession number :
180543837
Full Text :
https://doi.org/10.1080/15257770.2024.2421307