Single-Cell RNA Sequencing and Combinatorial Approaches for Understanding Heart Biology and Disease.

Simple Summary: Scientists have developed new techniques that allow them to study the heart at an incredibly detailed level, focusing on individual cells rather than looking at groups of cells together. This is important because the heart is made up of many different types of cells, each with its own role, and understanding how they interact is key to discovering what happens in both healthy hearts and in heart disease. By using methods that look at the genes, proteins, and other molecules inside single cells, researchers can uncover how cells communicate and change over time. These discoveries are helping scientists learn more about how the heart develops, how it stays healthy, and what goes wrong in heart diseases. This knowledge could lead to better treatments and new ways to diagnose heart conditions, helping people live longer, healthier lives. By directly measuring multiple molecular features in hundreds to millions of single cells, single-cell techniques allow for comprehensive characterization of the diversity of cells in the heart. These single-cell transcriptome and multi-omic studies are transforming our understanding of heart development and disease. Compared with single-dimensional inspections, the combination of transcriptomes with spatial dimensions and other omics can provide a comprehensive understanding of single-cell functions, microenvironment, dynamic processes, and their interrelationships. In this review, we will introduce the latest advances in cardiac health and disease at single-cell resolution; single-cell detection methods that can be used for transcriptome, genome, epigenome, and proteome analysis; single-cell multi-omics; as well as their future application prospects. [ABSTRACT FROM AUTHOR]