细胞周期相关基因的胶质母细胞瘤预后模型构建及 RFC2 的细胞增殖效应研究.

Objective To investigate the relationship of replication factor C subunit 2 (RFC2) with the prognosis of glioblastoma (GBM) and cell proliferation, as well as its underlying molecular pathway in GBM development. Methods Using bioinformatics methods, cell cycle genes were screened as independent prognostic factors for GBM. Combined with clinical indicators, a risk scoring model for GBM patients was established and validated. The target gene RFC2 was analyzed with GO, KEGG, and GSEA. U87 GBM cells at logarithmic growth stage were transfected with lentivirus and divided into different groups(control, ShRFC2 #1, and shRFC2#2 groups). qRT-PCR, Western blotting, Edu staining, and cloning assay were used to detect mRNA expression, protein expression, and cell proliferation. Results The expression of RFC2 was upregulated in GBM and showed an obvious upregulation trend with the increase of pathological grade of glioma. The analyses of gene function and pathway indicated that RFC2 was involved in the processes of sister chromosome segregation, chromosome segregation, organelle fission, and mitosis by promoting the transition of G1 to S phase during cell cycle. qRT-PCR and Western blotting showed that compared with the control group, the amount of mRNA and translated protein in the knockdowned groups decreased(P<0.000 1). The positive rate of Edu staining and the colony forming ability decreased (P<0.000 1, P<0.001). Conclusion RFC2 is highly expressed in glioblastoma and associated with pathological grade of glioma and poor prognosis of patients. It also promotes the cell proliferation function of glioblastoma. RFC2 may be a potential biomarker and therapeutic target for glioblastoma. [ABSTRACT FROM AUTHOR]