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Degradation and preservation of nitrites in whole blood.
- Source :
-
Forensic Science International . Nov2024, Vol. 364, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- Understanding the factors that influence nitrite degradation in whole blood and developing methods for its stable preservation are crucial for ensuring accurate and reliable forensic identification in cases of nitrite poisoning. This study systematically monitored nitrite degradation and changes in hemoglobin proportions across different initial nitrite concentrations and blood samples. It was revealed that high nitrite concentrations rapidly reduced deoxyhemoglobin levels within the first 15 minutes and subsequently reacted with oxyhemoglobin at a slower rate. Therefore, the proportions of these two hemoglobin forms are key factors in determining nitrite degradation rates. Regarding preservation, the study examined the effects of low temperatures (4°C and −20°C) and various preservatives (potassium ferricyanide, N-ethylmaleimide) on nitrite stability. The results indicate that adding 6.6 g/L potassium ferricyanide can rapidly eliminate all deoxyhemoglobin and reduce oxyhemoglobin proportions to below 60 %, enabling stable preservation of high nitrite concentrations in whole blood for over 30 days at −20°C. The efficacy of potassium ferricyanide was further validated in forensic-acquired postmortem heart blood samples. • High nitrite levels reduce deoxyhemoglobin and react with oxyhemoglobin in the blood. • The hemoglobin ratio is the critical factor determining the rate of nitrite degradation. • Potassium ferricyanide removes deoxyhemoglobin and reduces oxyhemoglobin to below 60 % in 15 minutes. • Potassium ferricyanide preserves high nitrite concentrations in whole blood at −20°C for over 30 days. • The efficacy of potassium ferricyanide was further validated in forensic-acquired postmortem heart blood samples. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NITRITES
*FORENSIC sciences
*HEMOGLOBINS
*FERRICYANIDES
*QUALITY control
Subjects
Details
- Language :
- English
- ISSN :
- 03790738
- Volume :
- 364
- Database :
- Academic Search Index
- Journal :
- Forensic Science International
- Publication Type :
- Academic Journal
- Accession number :
- 180492706
- Full Text :
- https://doi.org/10.1016/j.forsciint.2024.112232