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Isolation, identification, and evaluation of the characteristics of Streptomyces sp. as the probiotic strains applied in aquaculture.

Authors :
Tam Pham Thi
Hien Nguyen Thi Thu
Huyen Vu Thi Bich
Mai Le Thi Tuyet
Hai Le Minh
Binh Ta Thi
Van Dao Thi Hong
Phuong Vo Hong
Thoa Vu Kim
Le Dinh Thi Thu
Phuoc Man Hong
Source :
Israeli Journal of Aquaculture-Bamidgeh. 2024, Vol. 76 Issue 3, p85-95. 11p.
Publication Year :
2024

Abstract

The present study was conducted to select Streptomyces strains that exhibited characteristics of probiotics for application in aquaculture. Streptomyces were isolated from soil and sediment samples collected from various habitats such as riverside, shrimp ponds, pangasius ponds, mangrove forests, or estuaries along the coast of Vietnam. Two strains of Streptomyces (AG12.2 and ND10.1) were selected due to (1) their potent antimicrobial activity against common pathogens that cause serious diseases in shrimp and pangasius such as E. ictaluri, A. hydophila, V. harveyi, V. parahaemolyticus, and (2) their superior ability to produce several extracellular enzymes that strongly hydrolyze organic compounds such as starch, CMC, and skimmed milk, compared to other strains. AG12.2 and ND10.1 can tolerate salinity from 0 ppt to 5 ppt and pH from 3 to 8; however, their growth is most vigorous at salt concentrations and pH between 0 ppt to 2 ppt, and 7.2 to 8, respectively. In vivo evaluation on L. vannamei in this study shows that shrimp that were fed with diets containing AG12.2 and ND10.1 had WG (%) increasing by 1.59 times and 1.61 times respectively, DGW (g/day) increasing by 1.64 times, and FCR decreasing by 1.30 times and 1.35 times respectively. The shrimp treated with AG12.2 and ND10.1 were then challenged with V. parahaemolyticus, resulting in a survival rate increase of 3.9 times and 3.71 times, respectively. Phylogenetic analysis revealed that strains AG12.2 and ND10.1 belong to Streptomyces kunmingensis and Streptomyces angustmyceticus, respectively. The findings of the present study led to the conclusion that Streptomyces kunmingensis AG12.2 and Streptomyces angustmyceticus ND10.1 are excellent candidates for producing beneficial probiotics for aquaculture. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0792156X
Volume :
76
Issue :
3
Database :
Academic Search Index
Journal :
Israeli Journal of Aquaculture-Bamidgeh
Publication Type :
Academic Journal
Accession number :
180445981
Full Text :
https://doi.org/10.46989/001c.121927