胶体金免疫层析法高通量测定动物肌肉组织中多兽药残留.

[Objectives] The purpose of this paper was to develop a method for the high-throughput determination of 8 sulfonamides, 3 tetracyclines, 2 quinolones and trimethoprim drug residues in pork and chicken tissues by colloidal gold immunochromatographic assay(GICA). [Methods] Sulfonamides, tetracyclines, quinolones and trimethoprim in animal muscle tissues were extracted by phosphate buffer and diluted by phosphate buffer with Tween 20, binding to colloidal gold-labeled monoclonal antibodies and inhibiting the binding of the antibodies to the antigen on the nitrocellulose membrane test line(T-line), which resulted in a change of the color of the T-line. The qualitative determination of poly-veterinary drug residues in the samples was made by comparing the color shades of the T-line and control line(C-line). [Results] This test optimized the key factors such as pH value, antibody addition, antigen encapsulation, Tween 20 addition and type of lyophilized protectant. The limit of detection for sulfonamides, tetracyclines, quinolones and trimethoprim was 50, 100, 50 and 35 μg·kg-1, respectively, and the sensitivity ≥97%, the false positive rate ≤2%, the false negative rate ≤3%, and the method was consistent with the results detected by the existing standard method. [Conclusions] The method was simple with high sensitivity, high precision, short detection time and good stability. It could be used for high-throughput rapid detection of multi-veterinary drug residues in animal muscle tissue. [ABSTRACT FROM AUTHOR]