국화에서 전신발현 프로모터의 구명.

Chrysanthemum is the most popular ornamental plant, after roses and lilies. The cauliflower mosaic virus (CaMV) promoter, which remains the most widely used promoter in dicotyledons, is a very strong promoter with sufficient effects in most crops. However, weak expression has often been reported in Chrysanthemum. Therefore, we searched for constitutive promoters available in Chrysanthemum. Based on the transcriptome analysis data of Chrysanthemum, nine constitutively expressed genes were selected, and each promoter region (1.0–3.0 kb) was isolated by genome walking. Only two of the nine promoters expressed GUS in tobacco and chrysanthemums. The major motif of the CmERF promoter (U41, 2060 bp) was related to the regulation of ethylene (ERELEE4) or gibberellin (PYRIMIDINEBOXOSRAMY1 and WRKY71OS). Similarly, the motif of the CmGA2 ox promoter (U47, 1060 bp) also contained gibberellin signaling factors, such as PRIMIDINEBOXHVEPB1 and WRKY71OS. Both promoters showed strong systemic expression in tobacco using GUS staining. Although weaker than in tobacco, significant expression was confirmed in the flowers and stems in chrysanthemum. The results of the GUS activity assay using chrysanthemum transformants showed that the transgenic line (#12) containing the U47 promoter had higher expression in all tissues than that containing the 35S-CaMV promoter. The U41 promoter was found to have a higher expression than the 35S-CaMV promoter in the stem. [ABSTRACT FROM AUTHOR]