半体内和体外法评定8 种蛋白质饲料 的产气性能和发酵性能.

The aim of this study was to develop an in vitro two-step method to simulate the digestive process of donkeys and evaluate the nutritional value of 8 protein feeds using in vivo nylon bag and in vitro two-step methods. Three Xinjiang donkeys of similar weight and age were selected. In vivo and in vitro methods were employed to study dry matter degradation rates and fermentation parameters of the 8 protein feeds. The results showed that: ① comparison between in vivo and in vitro studies revealed that the dry matter degradation rates of fish meal, cottonseed meal, and sunflower meal were significantly higher in the in vitro method than in the in vivo method (P<0.05). ② In enzymatic digestion in vitro, the dry matter degradation rates of fish meal and soybean meal were significantly higher than the other 6 protein feeds (P<0.05). In microbial fermentation in vitro, blood meal and sunflower meal had significantly higher dry matter degradation rates than the other 6 protein feeds (P<0.05). ③ For gas production during fermentation, soybean meal had significantly higher total gas production than the other 7 protein feeds (P<0.05). ④ In vitro fermentation parameters showed that rapeseed meal and meat and bone meal had significantly higher pH values than the other 6 protein feeds (P<0.05). Ammonia nitrogen content was significantly higher in fish meal and blood meal than in the other 6 protein feeds (P<0.05). Acetic acid content in sunflower meal was significantly higher than in the other 7 protein feeds (P<0.05). In conclusion, using enzymatic digestion and microbial inoculation of donkey feces to simulate the digestive process of donkeys can reflect the real situation of protein feed degradation and digestion in donkeys. There are differences among different protein feeds, with soybean meal, blood meal, and fish meal showing higher dry matter degradation rates compared to other protein feeds. [ABSTRACT FROM AUTHOR]