Antiadipogenic activity of homoeopathic preparation of Chelidonium majus employing 3T3-L1 cell line as a model.

Adipocytes are derived from mesenchymal stem cells through the process of adipogenesis. Adipocyte metabolism is abnormal in a range of disorders like obesity, nutritional insufficiency, and diabetes. The 3T3-L1 preadipocytes are most often used to create adipocyte models, and they can be differentiated into adipocyte cells under the right conditions. Homeopathic preparation of Chelidonium majus (HPCM) 30cH in the management of Obesity and Type 2 diabetes mellitus (T2DM) has much evidence in clinical practice. The 3T3-L1 preadipocyte cells were differentiated into adipocytes by using a differentiation cocktail. Cells were treated with HPCM 30cH attenuation at the concentration of 0.5%, 1%, and 2% for 15 days. Oil O Red Staining was used to assess lipid droplet (LD) accumulation. In order to determine the lipid content in 3T3-L1 adipocytes, cells were dissolved in isopropanol and the absorbance values were measured. Images were captured and analysed using ImageJ software. We investigated the action of HPCM in obesity using the 3T3-L1 adipogenesis model employing Human Mesenchymal Stem Cells (huMSC's). After differentiation of adipocytes many LDs were formed in 3T3-L1 preadipocytes which can be compared with no lipid droplet in 3T3-L1 non-differentiated cells. Further, area of differentiated adipocytes was mapped and then compared for LD accumulation in control and HPCM to check its anti-adipogenic activity. A significant reduction in the accumulation of lipid droplets was seen in 0.5%, 1% and 2% concentration of HPCM as compared to control during the differentiation of 3T3-L1 preadipocytes into adipocytes. Excessive differentiation of cells and high fat accumulation in the adipose tissue are closely linked to obesity. Preadipocyte differentiation inhibitors may have preventive and therapeutic potential as anti-obesity drugs. HPCM 30cH has the potential to act as an antiadipogenic agent which can be used to combat various diseases like obesity, T2DM, a nd cardiovascular diseases. [ABSTRACT FROM AUTHOR]