Repurposing the anti-angiogenic activity of homoeopathic medicines by employing Chick embryo model-An In-Ovo Study.

Background: Homoeopathic medicines have the potential to treat patients suffering from cancers, Diabetic Retinopathy, and hemorrhagic disorders wherein anti-angiogenesis plays a vital role. Homoeopathic medicines have been used to treat hemorrhagic disorders. For example, Hamamelis is used in hemorrhages, varicose veins and venous congestion. Thalaspi bursa is used in the flabby conditions of uterus, hemorrhagic conditions of uterine fibroid. This being said, action of homoeopathic medicines remains unknown. Therefore, this study was intended to establish the angiogenic/anti-angiogenic effect of the selected homoeopathic medicines employing chick embryo model. Objective: To test toxicity and study the antiangiogenic properties of selected homoeopathic medicines employing YSM assay in the chick embryo model. Materials and Method: Yolk Sac Membrane is widely utilized as an in vivo system to study angiogenesis and anti-angiogenesis. The images for all YSMs were captured and analyzed for angiogenic/anti-angiogenic effect. Results: Significant antiangiogenic effects were observed at 10% concentrations of Hamamelis 200cH, Thalaspi bursa 200cH and Thalaspi bursa 1M. The reduction in vessel density and total vascular network length, total branching points, total networks and total segment symbolizes that these drugs have antiangiogenic potential and therefore may assist in the treatment of menstrual disorders, which are followed by cramps and discharge of clots. Conclusion: The action of selected homoeopathic medicines as anti-angiogenic agents is proven and can be used to combat various diseases like hemorrhages, varicose veins, in venous congestion, and problems related to hemorrhagic disorders. This will further help to study the action of these Homoeopathic medicines as a potential line of treatment in patient suffering from cancers, Diabetic Retinopathy, and hemorrhagic disorders wherein anti-angiogenesis plays a vital role. [ABSTRACT FROM AUTHOR]