YEŞİL SARIMSAK (ALLIUM SATIVUM L.) YAPRAKLARINDA BULUNAN FENOLİK VE ANTİOKSİDAN MADDELERİN EKSTRAKSİYON KOŞULLARININ YÜZEY YANIT YÖNTEMİ İLE OPTİMİZASYONU.

The demand for natural bioactive compounds and products that contain these compounds has increased because of the desire to live healthier. The Response Surface Methodology (RSM) was used to find the optimum parameters for getting the highest level of total phenolic compounds (TPC) and antioxidant compounds (AC) out of green garlic leaves (Allium sativum L.). In this study the ethanol concentration (60--80%), application time (15--45 min.), and product/solvent (P/S) ratio (1/50-- 1/100) were selected as independent variables. As responses, TPC and AC analyses were used. The study found that 69.70% ethanol, 15 min., and 1/50 U/S ratio were the optimum conditions. Under these conditions, the experimental TPC and AC values of the extracted samples were found to be 15.18±1.4 mg GAE/g DM and 88.16±1.18%, respectively. The fact that the experimental values were very close to RSM's expected values demonstrated that the models were suitable. [ABSTRACT FROM AUTHOR]