子宫内膜癌组织 miR-155, miR-202 表达与增殖侵袭基因表达, 临床病理参数和预后的关系.

Objective: To investigate the relationship between the expression of microRNA-155 (mi R-155) and microRNA-202 (mi R-202) and the expression of proliferation and invasion genes, clinicopathological parameters and prognosis in endometrial carcinoma (EC) tissue. Methods: 135 EC patients who were underwent surgical treatment in our hospital from October 2015 to October 2018 were selected as observation group, and 135 patients with uterine fibroids who were underwent total hysterectomy during the same period were selected as control group, the expression of mi R-155, mi R-202 and proliferation genes (Cdk4, Cdk6, Efemp2, DJ-1, RRM2) and invasion genes (Dkk1, Ezh2, HMGB1) in two groups were detected and compared by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), the relationship between the expression of mi R-155 and mi R-202 in EC tissues and the clinicopathological parameters of patients were analyzed. The 5 years cumulative survival rate of high expression and low expression of mi R-155 and mi R-202 were analyzed by Kaplan-Meier survival curve. Results: The relative expression levels of mi R-155, Ezh2, HMGB1, Cdk4,Cdk6, DJ-1 and RRM2 in observation group were higher than those in control group, and the relative expression levels of mi R-202, Dkk1and Efemp2 were lower than those in control group (P＜0.05). The relative expression of mi R-155 was positively correlated with Cdk4,Cdk6, DJ-1, RRM2, Ezh2 and HMGB1, and negatively correlated with Efemp2 and Dkk1 (P＜0.05). The relative expression of mi R-202was positively correlated with Efemp2 and Dkk1, and negatively correlated with Cdk4,Cdk6, DJ-1, RRM2, Ezh2 and HMGB1 (P＜0.05). The higher the FIGO stage of EC patients, the lower the degree of differentiation, the deeper the myometrial invasion and the higher the relative expression of mi R-155 in patients with lymph node metastasis, the lower the relative expression of mi R-202 (P＜0.05). The 5years overall survival rate in high mi R-155 group was 68.66%, which was lower than that 87.50% in low mi R-155 group (P＜0.05). The 5years survival rate in high mi R-202 group was 91.80%, which was higher than that 65.71% in low mi R-202 group (P＜0.05). Conclusion: The expression of mi R-155 increase and the expression of mi R-202 decrease in EC tissues, which are significantly correlate with the expression of proliferation and invasion genes, FIGO stage, differentiation degree, depth of myometrial invasion and lymph node metastasis, the higher the expression of mi R-155, the lower the expression of mi R-202, and the worse the prognosis of patients. [ABSTRACT FROM AUTHOR]