胶质瘤组织 miR-195, miR-3653 与临床病理特征及预后的关系.

Objective: To investigate the expression of mi R-195 and mi R-3653 in glioma tissue and to analyze their relationship with clinicopathological features and prognosis of glioma. Methods: Collected paraffin specimens of 42 glioma patients who underwent surgical resection at The Seventh Affiliated Hospital of Guangxi Medical University from January 2016 to June 2019 as the glioma group, and another 30 patients with traumatic brain injury who underwent surgical treatment during the same period were selected, and the paraffin specimens of the frontal or temporal lobe tissue removed during surgery were used as the control group. The relative expression of mi R-195 and mi R-3653 in glioma group and control group were detected by real-time fluorescent quantitative polymerization chain reaction (qRT-PCR), and the relationship between the expression of mi R-195 and mi R-3653 and the clinicopathological characteristics of glioma were analyzed. The relationship between mi R-195, mi R-3653 expression and prognosis in glioma patients was analysised by Kaplan Meier survival curve. The factors affecting the prognosis of glioma patients were analysised by Multivariate Cox regression. Results: The expression level of mi R-195 and mi R-3653 in glioma group were lower than those in control group (P＜0.001). There were significant differences in mi R-195 expression among patients with different pathological grades and distant metastasis (P ＜0.001). There were significant differences in mi R-3653 expression among patients with different tumor diameter,pathological grade and distant metastasis (P＜0.001). Kaplan-Meier survival curve showed that the three years survival rate of mi R-195and mi R-3653 low expression group were lower than those of mi R-195 and mi R-3653 high expression group (P＜0.05). Multivariate Cox regression analysis showed that distant metastasis, pathological grade III-IV level, low mi R-195 expression, and low mi R-3653expression were risk factors for the prognosis of glioma patients (P＜0.05). Conclusion: The low expression of mi R-195 and mi R-3653 in glioma patients is associated with distant metastasis, pathological grading of grade III-IV level, and low survival rate, mi R-195 and mi R-3653 may become prognostic indicators and therapeutic targets for glioma. [ABSTRACT FROM AUTHOR]