Actividad antifúngica in vitro de nanopartículas de plata a partir de hojas de Leea coccinea L. frente a Fusarium spp. y Penicillium spp.

The objective of this work was to determine the antifungal activity of three batches of AgNPs obtained from Leea coccinea L. on mycelial growth of Fusarium spp. and Penicillium spp. associated with common bean (Phaseolus vulgaris L.) and potato (Solanum tuberosum L.) seeds and spore germination of of Fusarium spp. Seven concentrations (0.485, 0.242, 0.121, 0.060, 0.03, 0,.015, and 0.007 mg/ml) of the AgNPs suspensions were evaluated using the serial dilution method. The Minimum Fungicide Concentration (MFC) of the AgNPs against the different microorganisms was determined. The results showed that the application of the AgNPs was effective in inhibiting growth of Fusarium spp. and Penicillium spp. The CMF of the AgNPs from the three batches ranged between 0.485 and 0.242 mg/ml against the phytopathogenic agents. The inhibition of mycelial growth of phytopathogens increased with increasing concentrations. The suspension of AgNPs from L. coccinea affected spore germination of the isolates of F. oxysporum and Fusarium spp. No. 1 in the two highest concentrations (0.485 and 0.242 mg/ml). It was confirmed that the higher the AgNPs concentration, the greater the antifungal effect on mycelial growth and spore germination of the phytopathogens studied. The results obtained denote the efficacy of this product as an alternative in the fight against phytopathogenic fungi. [ABSTRACT FROM AUTHOR]