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The generation of stable microvessels in ischemia is mediated by endothelial cell derived TRAIL.

The generation of stable microvessels in ischemia is mediated by endothelial cell derived TRAIL.

Authors :
Cartland, Siân P.
Patil, Manisha S.
Kelland, Elaina
Le, Natalie
Boccanfuso, Lauren
Stanley, Christopher P.
Cholan, Pradeep Manuneedhi
Dona, Malathi I.
Patrick, Ralph
McGrath, Jordan
Qian Peter Su
Alwis, Imala
Ganss, Ruth
Powell, Joseph E.
Harvey, Richard P.
Pinto, Alexander R.
Griffith, Thomas S.
Loa, Jacky
Aitken, Sarah J.
Robinson, David A.
Source :
Science Advances. 10/4/2024, Vol. 10 Issue 40, p1-17. 17p.
Publication Year :
2024

Abstract

Reversal of ischemia is mediated by neo-angiogenesis requiring endothelial cell (EC) and pericyte interactions to form stable microvascular networks. We describe an unrecognized role for tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in potentiating neo-angiogenesis and vessel stabilization. We show that the endothelium is a major source of TRAIL in the healthy circulation compromised in peripheral artery disease (PAD). EC deletion of TRAIL in vivo or in vitro inhibited neo-angiogenesis, pericyte recruitment, and vessel stabilization, resulting in reduced lower-limb blood perfusion with ischemia. Activation of the TRAIL receptor (TRAIL-R) restored blood perfusion and stable blood vessel networks in mice. Proof-of-concept studies showed that Conatumumab, an agonistic TRAIL-R2 antibody, promoted vascular sprouts from explanted patient arteries. Single-cell RNA sequencing revealed heparin-binding EGF-like growth factor in mediating EC-pericyte communications dependent on TRAIL. These studies highlight unique TRAIL-dependent mechanisms mediating neo-angiogenesis and vessel stabilization and the potential of repurposing TRAIL-R2 agonists to stimulate stable and functional microvessel networks to treat ischemia in PAD. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23752548
Volume :
10
Issue :
40
Database :
Academic Search Index
Journal :
Science Advances
Publication Type :
Academic Journal
Accession number :
180224045
Full Text :
https://doi.org/10.1126/sciadv.adn8760