lncRNA GIHCG 靶向 miR-429 对食管鳞状细胞癌 细胞增殖、迁移及侵袭的影响.

Objective To investigate the effects of long non-coding RNA (lncRNA) GIHCG targeting microRNA (miR)-429 on the proliferation, migration, and invasion of esophageal squamous cell carcinoma (ESCC) cells. Meth⁃ ods Human ESCC cell lines (EC9706, TE1, and KYSE150 cells) and normal esophageal squamous epithelial cell line (Het-1A cells) were routinely cultured. The expression levels of lncRNA GIHCG and miR-429 in the cells were detected by RT-PCR, and the experimental cells were screened. The experimental cells in the logarithmic growth phase were randomly divided into the si-NC group and the si-GIHCG group, which were transfected with lncRNA knockdown plasmid negative control and lncRNA GIHCG knockdown plasmid, respectively. The expression levels of lncRNA GIHCG and miR429 in cells were detected by RT-PCR. The proliferation ability of the cells [optical density (OD) value] was detected by CCK-8. The migration ability of the cells was detected by cell scratch test. The invasion ability of the cells was detected by Transwell chamber test. The expression levels of cell proliferation phenotype proteins [CyclinD1, proliferating cell nuclear antigen (PCNA)] and metastasis phenotype proteins [matrix metalloproteinase 2 (MMP-2), matrix metalloproteinase 9 (MMP-9)] in the cells were detected by Western blotting. The experimental cells in the logarithmic growth phase were randomly divided into the GIHCG-WT + miR-429 mimics group, the GIHCG-WT + NC-mimics group, the GIHCG-MUT + miR-429 mimics group, and the GIHCG-MUT + NC-mimics group, respectively. The targeted relationship between lncRNA GIHCG and miR-429 was verified by dual luciferase reporter gene assay. Results Compared with Het-1A cells, the relative expression levels of lncRNA GIHCG in EC9706, TE1 and KYSE150 were higher (all P<0. 05), and the relative expression levels of miR-429 were lower (all P<0. 05). Since the relative expression level of lncRNA GIHCG was the highest and the relative expression level of miR-429 was the lowest in TE1 cells, TE1 cells were used as the experimental cells. Compared with the si-NC group, the relative expression level of lncRNA GIHCG in the si-GIHCG group was lower, the relative expression level of miR-429 was higher, the OD values were lower, the percentage of cell movement distance was lower, and the relative expression levels of CyclinD1, PCNA, MMP-2 and MMP-9 were lower, and the differences were statistically significant (all P<0. 05). Compared with the GIHCG-WT + NC-mimics group, the relative luciferase activity in the GIHCG-WT + miR-429 mimics group was lower (P<0. 05); there was no statistically significant difference in the relative luciferase activity between the GIHCG-MUT + miR-429 mimics group and the GIHCG-MUT + NC-mimics group (P> 0. 05). Conclusions The lncRNA GIHCG is highly expressed and the miR-429 is low expressed in ESCC cells. Knockdown of lncRNA GIHCG inhibits the proliferation, migration and invasion of ESCC cells by targeting miR-429. [ABSTRACT FROM AUTHOR]