含白藜芦醇培养基培养的人眼结膜和胸部皮肤病理性 瘢痕成纤维细胞增殖凋亡侵袭迁移情况观察.

Objective To observe the proliferation, apoptosis, invasion, and migration of human ocular conjunctival pterygium (ocular pathological scar) fibroblasts and human anterior thoracic skin pathological scars (skin-derived pathological scars) fibroblasts cultured in resveratrol-containing medium, in order to investigate the effects of resveratrol on the proliferation, apoptosis, invasion, and migration of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts. Methods Primary human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts were isolated by tissue block adherent method. The cell viability (OD value) of human ocular and skin-derived pathological scar fibroblasts was detected by CCK-8 when the cells were cultured in media containing 0 (control), 1, 2, 4, 8, 16, 32, 64,128 and 256 µg/mL resveratrol for 48 h. Human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts were cultured with 0 and 75 µg/mL resveratrol for 8 h, and the apoptosis rate was measured by Tunel method. These two kinds fibroblasts were cultured in 0 and 30 µg/mL resveratrol media for 48 h, and the migration and invasion abilities of the cells (the number of invasive migratory cells) were observed. Results The OD values of human ocular pathological scar fibroblasts cultured in media containing 128 and 256 µg/mL resveratrol decreased (both P<0. 05), and the OD values of skin-derived pathological scar fibroblasts cultured in media containing 64, 128, and 256 µg/ mL resveratrol all decreased (all P<0. 05), in comparison with those at 0 µg/mL resveratrol. Compared with those at 0 µg/mL resveratrol, the apoptosis rates of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts cultured in 75 µg/mL resveratrol medium increased (all P<0. 05), and the number of invasion and migration cells of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts cultured in medium containing 30 µg/mL resveratrol became smaller (all P<0. 01) . Conclusions Human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts cultured in resveratrol-containing medium have reduced viability, invasion, and migration abilities, and increased apoptosis rate. Resveratrol can significantly inhibit the proliferation, invasion, and migration and promote the apoptosis of human ocular pathological scar fibroblasts and skin-derived pathological scar fibroblasts. [ABSTRACT FROM AUTHOR]