Evaluation of in vitro effects of platelet rich plasma on cadmium chloride–treated rat sperm.

Cadmium is a heavy metal with wide industrial applications. Humans are exposed to cadmium through different ways. Cadmium demonstrates a low excretion rate and accumulates in liver, kidney, and testes. Platelet rich plasma (PRP) contains various biologically active factors. This study aims at investigating the effect of PRP on incubated rat sperm parameters exposed to cadmium chloride. In this study, epididymal sperms were collected from 20 Wistar rats. The sperm suspension was divided into four groups: group 1 (control), group 2 (treated with cadmium chloride (Cd Cl2)), group 3 (treated with PRP and Cd Cl2), and group 4 (treated with PRP). The samples were incubated in room temperature for 1 h, and the assessments were made at 60 min after incubation. Sperm parameters including viability, abnormal morphology rate, membrane integrity, and chromatin condensation were evaluated. The results showed that viability and membrane integrity of sperms treated with CdCl2 (group 2) was significantly decreased compared to other groups. Supplementation of sperms with PRP improved viability of sperms in group 3. Abnormal morphology rate and chromatin condensation of sperms were decreased in group 3 in comparison to group 2. These results suggest beneficial effect of PRP on viability, morphology, membrane, and DNA integrity of sperms exposed to CdCl2. [ABSTRACT FROM AUTHOR]