In-vitro, anthelminthic effects of Azadirachta indica (Neem), Mallotus philippinensis (Kamala), and Melia azedarach (Bakain) leave extract against Ascaridia galli.

The present study was conducted to evaluate the anthelmintic activity of aqueous extracts of Azadirachta indica (Neem), Mallotus philippinensis (Kamala), Melia azedarach (Bakain), and their combination as herbal extracts against Ascaridia galli infection. To determine efficacy, egg hatch test (EHT) and adult motility assay (AMS) were performed using increasing concentrations of 12.5, 25, 50, and 100 mg/ml of aqueous plant extracts, in conjunction with 7.5 mg/ml Albendazole as the positive control and phosphate buffered saline (PBS) as the negative control for up to 15 h. There was 100% mortality achieved with the combined herbal extract (LC50 = 36.7 µg/ml), whereas mortality with extract from Mallotus 50 philippinensis (LC50 = 41.4 µg/ml), Melia azedarach (LC50 = 147 µg/ml), and 50 50 Azadirachta indica (LC50 = 189 µg/ml) were 87.5%, 62.5%, and 50% respectively, 50 at 15 hours post-exposure. These findings suggest that Azadirachta indica, Mallotus philippinensis, Melia azedarach herbal extracts, or their combination, should be further exploited to control Ascaridia galli infection. [ABSTRACT FROM AUTHOR]