Red‐Emitting Pyrazole Azo Hydrazone and Its Metal Complexes for Photophysical Probing, Latent Fingerprints, Anti‐counterfeiting, and Biological Studies.

ABSTRACT The present paper discusses the synthesis of unprecedented red‐emitting mono‐azo compound pyrazole azo fluorescent tag (PAFT) by a diazotization method, followed by an electrophilic substitution reaction. Further, the metal complex of PAFT is also prepared by refluxing the ethanolic solution of the ligand with the corresponding metal (RuCl3 and C4H6CuO4.H2O) salts under suitable experimental conditions. The synthesized fluorophore PAFT displays intense fluorescence in the aggregate state and has excellent qualities, including high purity, low cost, eco‐friendliness, and good photostability. These unique characteristics of PAFT led to the development of new fluorescence‐based technology for the in situ viewing of latent fingerprints. Various spectroscopic and analytical techniques are used to confirm the structural characteristics of the synthesized compounds, including UV–Vis, FT‐IR, (1H) NMR, HRMS, TGA, VSM, ESR, and powder XRD methods. The potency of the antibacterial activity of the compounds was evaluated. The results revealed that compound PAFT and [Ru(PAFT)Cl3] H2O showed excellent sensitivity at 0.8 μL/mL against the Gram‐positive bacteria Staphylococcus aureus. [ABSTRACT FROM AUTHOR]