خالص سازی فوزوباکتریوم نوکلئاتوم از پاکتهای پریودنتال دندانی.

Introduction: Fusobacterium nucleatum (F. nucleatum) is an anaerobic, gram-negative bacterium, morphologically an elongated bacillus with a sharp end belonging to the Bacteroidaceae. It is the normal flora of the mouth that is present in other organs in some diseases. Given the role of F. nucleatum in the creation and development of human diseases, it is very important to investigate its characteristics and functions. However, due to the obligate anaerobic nature of F. nucleatum, purification and long-term storage of the bacteria pose problems. The aim of this study is to find an optimal method for the purification of F. nucleatum to enable a detailed study of the functions and mechanisms of the bacteria to provide new therapeutic approaches to control or treat the diseases caused by F. nucleatum for future studies. Materials and Methods: Samples were taken from deep periodontal pockets of 16 patients using 40 size paper points with tryptic soy broth transfer medium (first method) and Columbia agar culture medium with gas pack type A and anaerobic jar (second method). The samples were then examined for the presence of F. nucleatum by macroscopic and microscopic observations, biochemical tests, PCR and sequencing. Results: The results showed that the tryptic soy broth transfer medium was not able to preserve and keep the bacteria alive, and the purified F. nucleatum was isolated by direct transfer to Columbia agar culture medium with gas pack type A and anaerobic jar. Discussion and Conclusion: Due to the obligate anaerobic nature of F. nucleatum, the use of transfer medium is not efficient for isolation due to the greater exposure of bacteria to air oxygen, and the optimal method for purification of F. nucleatum is the second sampling method. [ABSTRACT FROM AUTHOR]