Chemical constituents from the endophytic fungus <italic>Aspergillus</italic> sp. S3 of <italic>Hibiscus tiliaceus</italic>.

AbstractA new sterol, aspersterol E (<bold>1</bold>), a newly discovered alkaloid, asperginine A (<bold>2</bold>), and five known compounds (<bold>3–7</bold>) were obtained from the endophytic fungus <italic>Aspergillus</italic> sp. S3 of <italic>Hibiscus tiliaceus</italic> Linn. The compounds were extracted from their fermentation products using silica gel, ODS C18, and semi-preparative HPLC. The structure of each compound was determined through spectroscopic analysis. All the obtained compounds (<bold>1–7</bold>) were evaluated for their cytotoxic activity against the mouse pre-gastric cancer cell line MFC by using the MTT assay. The IC50 values of compounds <bold>1</bold>, <bold>2</bold>, <bold>3,</bold> and <bold>5</bold> were found to be 153.43 μM, 61.25 μM, 73.19 μM, and 181.69 μM respectively. [ABSTRACT FROM AUTHOR]