Back to Search Start Over

Comprehensive exploration of a traditional Chinese medicinal plant of Magnolia officinalis based on high-coverage mass spectrometry and multidimensional chemical-biological analysis.

Authors :
Wang, Wen-Yu
Song, Ya-Mei
Zhang, Jia-Nuo
Zhao, Ming-Yue
Pei, Wen-Han
Zhang, Hui
Yin, Hai-Bo
Xu, Zhi-Li
Xin, Gui-Zhong
Xie, Ming
Kang, Ting-Guo
Chen, Yue-Hua
Song, Hui-Peng
Source :
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Oct2024, Vol. 1246, pN.PAG-N.PAG. 1p.
Publication Year :
2024

Abstract

• A novel strategy was proposed for the comprehensive analysis of Magnolia officinalis. • A total of 103 compounds were identified from the whole plant of Magnolia officinalis. • Multidimensional chemical-biological analysis of Magnolia officinalis was established. • The 6 parts of Magnolia officinalis were explored and compared for the first time. • Magnolol and honokiol were found to be synergistic α-glucosidase inhibitors. Magnolia bark is a traditional Chinese medicine used for hypoglycaemia. With the widespread use of Magnolia bark, its resources are facing a serious shortage. To address this issue, a strategy based on high-coverage mass spectrometry (HCMS) and multidimensional chemical-biological analysis (MCBA) was proposed for the comprehensive exploration of Magnolia officinalis which is the main source of Magnolia bark. The strategy is divided into three main steps. In the first step, the stem bark, stem xylem, root bark, root xylem, leaf and rootlet of Magnolia officinalis were comprehensively analyzed using high-coverage mass spectrometry. In the second step, multivariate statistical analysis was used to explore the heterogeneity of the six parts and detect differential chemical components. In the third step, a combination of experimental screening and molecular docking was used to explore α-glucosidase inhibitors from Magnolia officinalis. Multidimensional chemical-biological analysis (MCBA) of Magnolia officinalis was achieved by combining the last two steps. Finally, a total of 103 compounds were identified from the whole plant of Magnolia officinalis. Differential components of stem bark, stem xylem, leaf, root bark, root xylem and rootlet were systematically revealed. A pair of positional isomers, namely magnolol and honokiol, were found to be α-glucosidase inhibitors. The activity of their combination is superior to that of each single compound, indicating that magnolol and honokiol are in a synergistic relationship. This strategy contributes to comprehensive exploitation of functional plants and effective alleviation of resource shortage. This study also provides a research paradigm for other similar traditional Chinese medicinal plants. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
15700232
Volume :
1246
Database :
Academic Search Index
Journal :
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
Publication Type :
Academic Journal
Accession number :
179602544
Full Text :
https://doi.org/10.1016/j.jchromb.2024.124290