DIAGNOSIS OF SARS-COV-2 VIRUS WITH REAL TIME PCR IN AMBEDKAR NAGAR DISTRICT OF UTTAR PRADESH: A NEAR CARE APPROACH.

Background: Coronavirus disease 2019 (COVID-19) is an emerging human-to-human infectious disease that broke out in early December 2019 and threatens global public health, causing widespread concern. This respiratory disease is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The development of reliable techniques for COVID-19 diagnosis is a significant step to prevent further infection. Thus in this study we see the prevalence of Covid 19 cases in eastern part of Uttar Pradesh by a molecular diagnostic method. Aim and Objective: To study the diagnosis of sars-cov-2 virus with real time PCR in patients attending the tertiary care centre. Material and Methods: This was a Retrospective study carried out in the Department of Microbiology between September 2020 to June 2022. The Samples were collected from COVID19 clinically suspected patients at GMC, Ambedkarnagar and evaluated with real time reverse transcriptase polymerase chain reaction (RT-PCR) tests. Also few samples were sent for genome sequencing and analysed. Results: In our study a total of 2999 patients were diagnosed with COVID-19. Five samples showed emerging delta strain in mid part of study. Conclusion: RT-PCR amplification of viral nucleic acid has been widely recognized as the gold standard for diagnosis of COVID-19 and can effectively confirm timely and accurate SARS-CoV2 infection. [ABSTRACT FROM AUTHOR]