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OPTIMIZATION OF in vitro PROTOCOL FOR SURVIVAL AND REGENERATION OF GAMMA-INDUCED SCALPS IN Musa acuminata cv. Calcutta-4.
- Source :
-
South-Western Journal of Horticulture Biology & Environment . Jul2024, Vol. 15 Issue 1, p19-38. 20p. - Publication Year :
- 2024
-
Abstract
- This study aimed to investigate the impact of varying gamma irradiation doses on banana scalps' survival and regeneration potential, specifically focusing on their interaction with different culture media components. Banana scalps, derived from a selected cultivar (Calcutta-4), were exposed to gamma irradiation doses ranging from 10 Gy to 70 Gy. Following irradiation, the scalps were transferred to fresh culture media, and their survival rates and regeneration potential were assessed. The findings revealed a clear inverse relationship between radiation dose and scalp survival. Lower doses of 10 Gy and 20 Gy exhibited higher survival rates when cultured on a modified MS medium. In comparison, higher doses, such as 50 Gy and 60 Gy, led to a significant decrease in survival. The optimal gamma irradiation dose for both scalp survival and successful regeneration fell within the range of 30 Gy to 40 Gy when using a medium supplemented with lower concentrations of BAP and Ads (1.5 mg/l to 2.0 mg/l BAP + 200 mg/l to 300 mg/l Ads). Furthermore, the study demonstrated that the surviving irradiated scalps exhibited a high potential for shoot regeneration when cultured on an MS medium with 3.0 mg/l BAP + 400 mg/l Ads. Gamma irradiation facilitated the initiation of new shoots, and the specific concentration of plant growth regulators and media composition influenced the response. These findings have significant implications for optimizing tissue culture techniques and improving crop production in banana cultivars and are crucial for advancing banana farming practices. [ABSTRACT FROM AUTHOR]
- Subjects :
- *AGRICULTURE
*MASS media influence
*TISSUE culture
*SCALP
*SURVIVAL rate
*BANANAS
Subjects
Details
- Language :
- English
- ISSN :
- 20679874
- Volume :
- 15
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- South-Western Journal of Horticulture Biology & Environment
- Publication Type :
- Academic Journal
- Accession number :
- 179548221