Impact of ventilation system with sucrose doses and wavelength on biomass production and arbutin content in Origanum majorana L. plantlets.

• In vitro plantlets of Origanum majorana exhibit photomixotrophic cultivation. • The sucrose concentration affected the in vitro accumulation of arbutin. • Ventilation and wavelength affected the growth, pigments and arbutin content. • Arbutin accumulation was higher in the 30%B:70%R and where there is a red light. • Monochromatic light blue, green and yellow have lower arbutin content. The ventilation system using porous membranes allows for better plantlet growth and increased survival during the acclimatization phase. The objective was to investigate the effect of alternative membrane system and wavelength on the growth and accumulation of arbutin in vitro plantlets of Origanum majorana. Nodal segments containing a pair of leaves were cultivated in vitro using MS culture medium and an alternative membrane system consisting of four porous membranes. Sucrose was added at concentrations of 0, 7.5, 15, and 30 g. Another experiment was conducted using four porous membranes supplemented with 15 g l -1 of sucrose and under different light-emitting diodes (LEDs): white LED (W); blue (B); green (G); yellow (Y); red (R); a combination of blue and red (50%B:50%R; 30%B:70%R; 70%B:30%R). Marjoram (Origanum majorana) plantlets exhibit photomixotrophic growth, with a medium containing 15 g of sucrose, and an alternative membrane system with 4 porous membranes promoting increased growth. The sucrose concentration influenced the accumulation of arbutin. The wavelength, in conjunction with the use of a membrane system, affected the growth, concentration of photosynthetic pigments, and accumulation of arbutin in marjoram plantlets. The highest biomass accumulation and arbutin synthesis were induced by the 30%B:70%R spectrum combination, while lower biomass and accumulation were observed under the monochromatic blue spectrum. Improving factors such as ventilation, wavelength, and sucrose can lead to more efficient resource use, reducing costs and increasing the yield of arbutin in vitro. [Display omitted] [ABSTRACT FROM AUTHOR]