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Plasticity-induced actin polymerization in the dendritic shaft regulates intracellular AMPA receptor trafficking.

Authors :
Wong, Victor C.
Houlihan, Patrick R.
Hui Liu
Walpita, Deepika
DeSantis, Michael C.
Zhe Liu
O'Shea, Erin K.
Source :
eLife. 8/15/2024, p1-39. 39p.
Publication Year :
2024

Abstract

AMPA-type receptors (AMPARs) are rapidly inserted into synapses undergoing plasticity to increase synaptic transmission, but it is not fully understood if and how AMPAR-containing vesicles are selectively trafficked to these synapses. Here, we developed a strategy to label AMPAR GluA1 subunits expressed from their endogenous loci in cultured rat hippocampal neurons and characterized the motion of GluA1-containing vesicles using single-particle tracking and mathematical modeling. We find that GluA1-containing vesicles are confined and concentrated near sites of stimulation-induced structural plasticity. We show that confinement is mediated by actin polymerization, which hinders the active transport of GluA1-containing vesicles along the length of the dendritic shaft by modulating the rheological properties of the cytoplasm. Actin polymerization also facilitates myosin-mediated transport of GluA1-containing vesicles to exocytic sites. We conclude that neurons utilize F-actin to increase vesicular GluA1 reservoirs and promote exocytosis proximal to the sites of synaptic activity. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
2050084X
Database :
Academic Search Index
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
179519040
Full Text :
https://doi.org/10.7554/eLife.80622