Gene Cloning and Bioinformatics Analysis of phoR Gene from Vibrio alginolyticus HY9901.

PhoR is a histidine kinase in a two-component regulatory system that regulates phosphorus metabolic pathways and undertakes the key mission of information transmission in pathogenic bacteria. The full-length phoR gene was successfully cloned from the Vibrio alginolyticus HY9901 strain. A comprehensive analysis of the cloned gene was conducted using bioinformatics. Sequence analysis revealed that the total length of the phoR gene (GenBank accession No.: KJ958404.1) is 1299 bp, with the coding region containing a total of 432 amino acid residues. The phylogenetic tree of PhoR revealed that it belongs to the same subclade as V. diabolicus. The SMART program was employed for the purpose of functional domain prediction, which revealed that PhoR possesses three major functional domains: PAS (amino acids 98 -166), His-KA (amino acids 205-272), and HATPase_c (amino acids 317429). [ABSTRACT FROM AUTHOR]