二十二碳六烯酸抑制人结肠癌细胞系 HT-29 增殖.

Objective To investigate the effect of docosahexaenoic acid (DHA) on human colon cancer cell line HT-29 and underlying mechanism. Methods Human colon cancer cell line HT-29 was incubated with DMSO (control), DHA (25, 50, 100 µmol/L) and 100 µmol/L DHA and/or 30 µmol/L 740Y-P. Proliferation was examined by MTT; apoptosis was detected by annexin V-FITC/PI. Western blot was used for detection of protein expression of Bcl-2, Bax apoptosis-related protein and PI3K/Akt/mTOR pathway, and RT-qPCR was used for checking mRNA expression of NLRP3/Caspase-1/IL-1ẞ pathway. Results Compared with the control group, DHA 25, 50, and 100 µmol/L treatment of HT-29 cells resulted in decreased cell survival (P < 0.05), increased apoptosis P < 0.05 ), decreased Bcl-2/Bax ratio P < 0.05 ) and decreased phosphorylation of PI3K, Akt and mTOR in HT-29 cells P < 0.05 or P < 0.01 . Expressions of NLRP3, Caspase-1 and IL-1ẞ mRNA were decreased (P < 0.05) . In addition, cell viability, protein phosphorylation (p-PI3K, p-Akt, p-mTOR) and relative mRNA expression of NLRP3, Caspase 1, and IL-1ẞ were lower in HT-29 cells which were co-incubated with DHA 100 µmol/L and 740Y-P 30 µmol/L than those in the control group ( P < 0.05 or P < 0.01 ) and 740Y-P 30 µmol/L group (P < 0.05), while higher than that of DHA 100 µmol/L group ( P < 0.05 or P < 0.01 ) . Conclusions DHA inhibits the proliferation of human colon cancer cell line HT-29, its mechanism is potentially related to the inhibition of PI3K/Akt/mTOR and NLRP3/Caspase-1/IL-1ẞ signaling pathways. [ABSTRACT FROM AUTHOR]