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A liquid culture method assisted by ATP analysis for accelerating laboratory experiments on ultraviolet disinfection of airborne bacteria.

Authors :
Guo, Kangqi
Wan, Zhishang
Jiang, Yi
Ho, Kin-Fai
Chen, Chun
Source :
Journal of Hazardous Materials. Oct2024, Vol. 478, pN.PAG-N.PAG. 1p.
Publication Year :
2024

Abstract

The solid culture method for measuring the efficiency of ultraviolet (UV) disinfection of airborne bacteria is time-consuming, typically taking 12–48 h. To expedite such experiments, this study proposed a liquid culture method assisted by adenosine triphosphate (ATP) analysis, as a liquid culture is faster than a solid culture, and measurement of ATP does not require waiting for visible colonies to form. Escherichia coli (E. coli) was used as the experimental bacterium. This study first compared the log reduction of bacteria in liquid as measured by the proposed method and by the traditional solid culture method. The minimum liquid culture time was determined for different bacterial concentration ranges. Finally, the feasibility of the proposed method was validated by UV disinfection experiments on airborne bacteria. The results indicated that the proposed method measured a similar log reduction to that of the solid culture method in liquid experiments. The minimum liquid culture time for E. coli in 105-106 colony forming units (CFU)/mL was 2 h. The validation experiments demonstrated that the proposed method is capable of measuring the UV disinfection efficiency of airborne bacteria. The proposed method can accelerate laboratory experiments on UV disinfection of airborne bacteria, which in turn can support the effective design and utilization of UV disinfection in real life. [Display omitted] • A liquid culture assisted by adenosine triphosphate analysis method was proposed. • The minimum liquid culture time was determined for different concentration ranges. • The proposed method can be used to measure UV disinfection of airborne bacteria. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03043894
Volume :
478
Database :
Academic Search Index
Journal :
Journal of Hazardous Materials
Publication Type :
Academic Journal
Accession number :
179418882
Full Text :
https://doi.org/10.1016/j.jhazmat.2024.135518