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Experimental observation of cavity-free ice-free isochoric vitrification via combined pressure measurements and photon counting x-ray computed tomography.
- Source :
-
Cryobiology . Sep2024, Vol. 116, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- Isochoric (constant-volume or volumetrically confined) vitrification has shown potential as an alternative cryopreservation-by-vitrification technique, but the complex processes at play within the chamber are yet poorly characterized, and recent investigations have prompted significant debate around whether a truly isochoric vitrification process (in which the liquid remains completely confined by solid boundaries) is indeed feasible. Based on a recent thermomechanical simulation of a high-concentration Me 2 SO solution, Solanki and Rabin (Cryobiology , 2023 , 111 , 9–15.) argue that isochoric vitrification is not feasible, because differential thermal contraction of the solution and container will necessarily drive generation of a cavity, corrupting the rigid confinement of the liquid. Here, we provide direct experimental evidence to the contrary, demonstrating cavity-free isochoric vitrification of a ∼3.5 M vitrification solution by combined isochoric pressure measurement (IPM) and photon-counting x-ray computed tomography (PC-CT). We hypothesize that the absence of a cavity is due to the minimal thermal contraction of the solution, which we support with additional volumetric analysis of the PC-CT reconstructions. In total, this study provides experimental evidence both demonstrating the feasibility of isochoric vitrification and highlighting the potential of designing vitrification solutions that exhibit minimal thermal contraction. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00112240
- Volume :
- 116
- Database :
- Academic Search Index
- Journal :
- Cryobiology
- Publication Type :
- Academic Journal
- Accession number :
- 179397742
- Full Text :
- https://doi.org/10.1016/j.cryobiol.2024.104935