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Urinary Proteomic Biomarkers of Trabecular Bone Volume Change during Army Basic Combat Training.
- Source :
-
Medicine & Science in Sports & Exercise . Sep2024, Vol. 56 Issue 9, p1644-1654. 11p. - Publication Year :
- 2024
-
Abstract
- Changes in bone microarchitecture resulting from chronic physical stress such as that experienced during U.S. Army Basic Combat Training (BCT) underscore its adaptive potential. There is a need for noninvasive biomarkers of adaptive bone formation that can be obtained at scale in real-world settings. Differential mass spectrometry (dMS), a label-free quantitative proteomics technique, may provide useful information about bone adaptation based on the proteomic features of urine. Purpose: The purpose of this study is to optimize a dMS-based urinary proteomic technique and evaluate the relationship between urinary proteome content and adaptive changes in bone microarchitecture during BCT. Methods: Urinary proteomes were analyzed with an optimized dMS technique in two groups of 13 recruits (N = 26) at the beginning (Pre) and end (Post) of BCT. Matched by age (21 ± 4 yr), sex (16 W), and baseline tibial trabecular bone volume fractions (Tb.BV/TV), these groups were distinguished by themost substantial (High) and minimal (Low) improvements in Tb.BV/TV. Differential protein expression was analyzed with mixed permutation ANOVA and false discovery proportion-based adjustment for multiple comparisons. Results: Tibial Tb.BV/TV increased from pre- to post-BCT in High (3.30 ± 1.64%, P < 0.0001) but not Low (-0.35 ± 1.25%, P = 0.4707). The optimized dMS technique identified 10,431 peptides from 1368 protein groups that represented 165 integrative biological processes. Seventy-four urinary proteins changed from pre- to post-BCT (P = 0.0019), and neutrophil-mediated immunity was the most prominent ontology. Two proteins (immunoglobulin heavy constant gamma 4 and C-type lectin domain family 4 member G) differed from pre- to post-BCT in High and Low (P = 0.0006). Conclusions: The dMS technique can identify more than 1000 urinary proteins. At least 74 proteins are responsive to BCT, and other principally immune system--related proteins show differential expression patterns that coincide with adaptive bone formation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01959131
- Volume :
- 56
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Medicine & Science in Sports & Exercise
- Publication Type :
- Academic Journal
- Accession number :
- 179290724
- Full Text :
- https://doi.org/10.1249/MSS.0000000000003464