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An improved method for tubulin staining of ciliated eukaryotes.
- Source :
-
Water Biology & Security . Jul2024, Vol. 3 Issue 3, p1-7. 7p. - Publication Year :
- 2024
-
Abstract
- Ciliated microeukaryotes (ciliates) are distinguished by their cilia, which are rich in tubulin. We developed a method for tubulin staining in ciliate cells that involves using live-cell tubulin-staining dyes instead of antibodies thereby streamlining the staining process, which is effective across diverse ciliate lineages. Moreover, our method allows integration with immunofluorescence staining using antibodies when needed. The potential applications of this technique extend to cell biology and ciliate morphological and ecological studies. [ABSTRACT FROM AUTHOR]
- Subjects :
- *TUBULINS
*CYTOLOGY
*EUKARYOTES
Subjects
Details
- Language :
- English
- ISSN :
- 27727351
- Volume :
- 3
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Water Biology & Security
- Publication Type :
- Academic Journal
- Accession number :
- 179290567
- Full Text :
- https://doi.org/10.1016/j.watbs.2024.100274