冬播甘蓝型油菜生育期进程及生理生化特性.

【Objective】The study aimed to solve the problem of inconsistent flowering periods in hybride production between winter rapeseed and spring rapeseed, thus providing a basis for the utilization of advantages of hybrids between winter rapeseed and spring rapeseed with strong winter hardiness in China.【Method】The strong winter rapeseed (NTS211, NTS304) and spring rapeseed (C05, C19) were used as materials. The seeds were sown in autumn (October 11th), and winter (December 10th, December 30th, January 19th, February 8th and February 28th) . The growth period, emergence rate, and reproductive performance were recorded, and the physiological and biochemical characteristics of leaves in different growth period were analyzed.【Result】The seeds sown in autumn (October 11th) emerged before winter, strong winter rapeseed could safely overwinter, while the overwintering rate of spring rapeseed was 0. The seeds sown after December 10th did not germinate during the winter period. In the early spring of the following year (March 21st to April 6th), after the temperature warmed up, the seeds began to germinate, with a germination ranging from 21.0% to 72.3%. The flowering period of winter-sown winter rapeseed was from June 21st to July 5th and July 3rd to July 20th. The flowering period of winter-sown spring rapeseed was from May 10th to May 12th and May 25th to May 30th. The flowering period of winter-sown winter rapeseed were from May 5th to May 30th. The flowering periods of autumn-sown winter rapeseed and winter-sown spring rapeseed coincided, and the longest overlap time of flowering periods between winter-sown (January 19th) spring rapeseed and autumn-sown winter rapeseed ware up to 18 days. With the delay of sowing time in winter, the flowering process of winter rapeseed was delayed, resulting in an extended growth period. However, the flowering process of spring rapeseed was advanced, leading to a shortened growth period. The fruiting performance of winter-sown winter rapeseed showed a downward trend, while the thousand-seed weight of winter-sown spring rapeseed showed an upward trend. Winter-sown winter rapeseed and spring rapeseed showed an upward trend in gibberellin content during the seedling stage, a downward trend during the bud stage, and a trend of initially increasing and subsequently decreasing during the flowering and silique stages. In the seedling stage, the auxin content of winter-sown winter rapeseed was significantly higher than that of spring rapeseed. The peroxidase activity during the bud stage was significantly higher than that in other growth stages. The content of malondialdehyde was the highest during the seedling stage, and the content of soluble protein was the highest during the flowering stage.【Conclusion】It is feasible to adjust the flowering period by sowing winter rapeseed in autumn and spring rapeseed in winter. The longest overlapping period of flowering between winter-sown spring rapeseed and autumn-sown winter rapeseed are 18 days. Winter sowing may affect the antioxidant enzymes, malondialdehyde, osmotic regulatory substances, and endogenous hormones in the leaves of Brassica napus, ultimately influencing its growth, development and fruiting performance. [ABSTRACT FROM AUTHOR]