Targeting the WWP1 gene with incensole acetate nanoemulsion: A novel therapeutic strategy for breast cancer.

The use of natural substances in cancer treatment is gaining popularity due to their potentially lower side effects compared to radiation or chemotherapy. The development of these substances for the treatment of cancer has been successful. New technologies now incorporate nanoformulations for nanomedicines that are intended to increase the anticancer potential of drugs synthesized from phytocompounds. Essential oil from Catharanthus roseus plant was extracted and incorporated into IA-NE and assess anticancer activity of synthesized formulation against breast cancer. The 9, 10-dimethylbenz[a] anthracene (DMBA) induced rat mammary tumor model was used to evaluate anticancer properties of biosynthesized nanoemulsion Nanoemulsions was examined for in vitro cytotoxicity activity, anti-cancer effects, and physicochemical characteristics. Study findings demonstrated that incensole acetate mediated nanoemulsion may successfully improve tumor parameters, oxidative stress enzyme levels and over expressed WWP1 level in DMBA induced rats. The present investigations indicate the effect of nanoemulsion to reduce expression level of WWP1 gene significantly in breast cancer induced model. Therefore, this nanoformulation is considered a key therapeutic agent for future cancer treatment, pending extensive molecular studies. [Display omitted] • Formulation and characterization of nanoformulation was performed by interpreting its UV, SEM, Zeta size and PDI. • Investigate the effects of synthesized nanoemulsion on DMBA tumor parameters. • Analysis of oxidative stress markers in cancer induced animals as treated with nanoemulsion. • Optimized nanoemulsions had a significant cytotoxicity against MBA-MB-231 cancer cell lines. • Investigation of WWP1 gene expression in cancer induced animals as treated with nanoemulsion. [ABSTRACT FROM AUTHOR]