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Mapping and functional analysis of regulatory sequences in the mouse λ5-VpreB1 domain
- Source :
-
Molecular Immunology . Jul2005, Vol. 42 Issue 11, p1283-1292. 10p. - Publication Year :
- 2005
-
Abstract
- Abstract: The λ5 and VpreB genes encode the components of the surrogate light-chain which forms part of the pre-B cell receptor and plays a key role in B cell development. In the mouse, the λ5 and VpreB1 genes are closely linked and are co-regulated by a multi-component locus control region. To identify the sequences that regulate λ5 and VpreB1 expression during B cell development, we have comprehensively mapped the DNaseI hypersensitive sites (HS) in the λ5-VpreB1 functional domain. The active domain contains 12 HS that are distributed at high density across the 18.3kb region that forms the λ5 and VpreB1 functional unit. Analysis of a reporter gene driven by the VpreB1 promoter in transgenic mice identified a novel enhancer associated with two HS located upstream of λ5. The λ5-VpreB1 locus was also found to be closely linked to the ubiquitously expressed Topoisomerase-3β (Topo3β) gene. The VpreB1 and Topo3β genes have entirely different expression patterns despite the fact that the two promoters are separated by a distance of only 1.5kb. [Copyright &y& Elsevier]
- Subjects :
- *TRANSGENIC mice
*B cells
*CELL membranes
*CELL receptors
Subjects
Details
- Language :
- English
- ISSN :
- 01615890
- Volume :
- 42
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Molecular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 17915770
- Full Text :
- https://doi.org/10.1016/j.molimm.2005.01.003