In-depth metaproteomics analysis reveals the protein profile and metabolism characteristics in pork during refrigerated storage.

Alterations in microbiotas and endogenous enzymes have been implicated in meat deterioration. However, the factors that mediate the interactions between meat quality and microbiome profile were inadequately investigated. In this study, we collected pork samples throughout the refrigeration period and employed metaproteomics to characterize both the pork and microbial proteins. Our findings demonstrated that pork proteins associated with the catabolic process are upregulated during storage compared to the initial stage. Pseudomonas , Clostridium , Goodfellowiella , and Gonapodya contribute to the spoilage process. Notably, we observed an elevated abundance of microbial proteins related to glycolytic enzymes in refrigerated pork, identifying numerous proteins linked to biogenic amine production, thus highlighting their essential role in microbial decay. Further, we reveal that many of these microbial proteins from Pseudomonas are ribosomal proteins, promoting enzyme synthesis by enhancing transcription and translation. This study provides intrinsic insights into the underlying mechanisms by which microorganisms contribute to meat spoilage. [Display omitted] • In-depth metaproteomics revealed key microbial metabolism affecting meat quality. • Innovatively proposed key role of microbial glycolytic enzymes in pork spoilage. • Enzyme synthesis of Pseudomonas and allies is notably enhanced during pork storage. • WGCNA reveals key proteins potentially involved in biogenic amines production. • Insights into interplay between host and microbial proteins during meat storage. [ABSTRACT FROM AUTHOR]