miR-888-5p对非小细胞肺癌细胞增殖、侵袭 能力的促进作用及其机制.

Objective To observe the effects of miR-888-5p on the proliferation and invasion abilities of non-smallcell lung cancer (NSCLC) cells, and to explore the possible mechanism. Methods Real-time fluorescence quantitative PCR was used to detect the relative expression levels of miR-888-5p in NSCLC cell lines (A549, NCI-H1299, PC-9, LLC, and NCI-H1650) and human normal lung epithelial cell line (BEAS-2B), . A549 cells with the most significant changes in the miR-888-5p relative expression were selected for subsequent experiments. A549 cells were divided into the miR-888-5p mimics group, miR-888-5p inhibitor group, and scramble group, which were transfected with miR-888-5p mimics, miR-888-5p inhibitor, and scramble sequences, respectively. Real-time fluorescence quantitative PCR was used to detect the relative expression level of miR-888-5p in cells. MTT assay was used to detect the cell proliferation abilities at 0, 24, 48, and 72 h of culture. Transwell chamber invasion assay was used to detect the cell invasion ability (expressed as the number of invading cells), . StarBase online prediction website predicted that the target gene of miR-888-5p was Tob1, which was verified through luciferase assay. Western blotting was used to detect the relative expression levels of Tob1, E-cadherin, vimentin proteins, p-JAK2/JAK2, and p-STAT3/STAT3 in cells. Results The relative expression levels of miR-888-5p in the miR-888-5p mimics group, miR-888-5p inhibitor group, and scramble group were 48. 9 ± 1. 78, 1. 06 ± 0. 24, and 8. 23 ± 0. 90, respectively, with statistically significant difference between groups (all P<0. 05), . Over the time of culture, the proliferation abilities of the three groups showed an increasing trend. At the same time point, the cell proliferation abilities and the invasive cells decreased in the miR-888-5p mimics group, scramble group, and miR888-5p inhibitor group in turn, with statistically significant difference between groups (all P<0. 05), . The relative expression levels of Tob1 and E-cadherin proteins in the miR-888-5p mimics group, scramble group, and miR-888-5p inhibitor group increased sequentially, while the relative expression levels of vimentin protein and p-JAK2/JAK2, p-STAT3/STAT3 decreased sequentially (all P<0. 05), . Conclusion MiR-888-5p can enhance the proliferation and invasion abilities of NSCLC cells, and their mechanism may be related to the activation of JAK2/STAT signaling pathway and targeted downregulation of Tob1 expression, thereby promoting the epithelial-mesenchymal transition. [ABSTRACT FROM AUTHOR]