濒危兰科植物三蕊兰根内生真菌的 分离鉴定及促生作用.

[Objective] The purpose of the paper was to study the diversity and growth promoting potential of culturable endophytic fungi in Neuwiedia singapureana, and provide a reference for the exploration of endophytic fungi resources in Hainan of China. (Method) The endo- phytic fungi strains of N. singapureana rooted from the Dialuoshan Nature Reserve in Hainan province were identified by culture method, combined with ITS sequencing, and the phosphorus dissolving and growth promotion ability of some fungi was tested. The endophytic fungi was co-cultured with the protocorms of D. officinale and A. rigida, to test their effects on the growth of the two orchids. (Result) By free- hand sections and microscopic observation, the fungal hypha in the cortex of root of the N. singapureana could be clearly observed. 24 OTUS were obtained, belonging to nine families and 14 genera, among which Trichoderma in Moniliaceae family had the highest isolation rate, with five genera (eg. Cylindrocarpon, Gliocephalotrichum) strains in Nectriaceae followed. Among them, five strains showed different degrees of phosphorus-decompressing ability, contaning Apiotrichum sp. (Neu242), Cylindrocarpon spp. (Neu83 and Neul54), Gliocladiopsis sp. (Neu245) and Mucor sp. (Neul23). Morover, Mucor sp. Neu123 and Apiotrichum sp. Neu242, showed significantly promoting effects on the growth of D. officinale and A. rigida seedlings. [Conclusion] There are abundant culturable fungi in the roots of N. singapureana. The endophytic fungi with potential phosphorus-soluble ability are screened from N. singapureana roots, which can provide growth-promoting strain resources for the artificial breeding of orchids, and also lay a foundation for the research on the mechanism of orchid mycorrhiza symbiotic phosphorus nutrition. [ABSTRACT FROM AUTHOR]