Problems in the assembly and analysis of the kangaroopox virus-NSW isolate.

The article focuses on discrepancies in the genome sequences and analysis of the Eastern grey kangaroo poxvirus strain New South Wales (EKPV-NSW) as reported by Sarker et al. It identifies errors in sequence alignment, nucleotide composition, recombination predictions, and gene annotations, suggesting that assembly mistakes and analytical errors led to incorrect conclusions about the genetic differences between EKPV-NSW and other kangaroopox virus strains.