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Effects of 10-MDP calcium salt on osteoblasts and fibroblasts.

Authors :
Zhou, Lvhui
Wu, Yumin
Bai, Zehua
Bian, Jingjing
Xie, Haifeng
Chen, Chen
Source :
Dental Materials. Sep2024, Vol. 40 Issue 9, p1322-1331. 10p.
Publication Year :
2024

Abstract

10-methacryloyloxidecyl dihydrogen phosphate monomer (10-MDP) is commonly used as a bonding monomer in universal adhesives. Adhesives that contain this monomer can directly contact the surrounding periodontium due to the chemical binding of 10-MDP with hydroxyapatite in hard tissue to form calcium salts. However, the effect of these calcium salts on the periodontium in the case of subgingival fillings remains poorly understood. The objective of this study was to investigate effects of 10-MDP calcium salts on osteoblasts and fibroblasts in the periodontal tissues. This study investigated the effects of different concentrations of 10-MDP calcium salts on the migration, proliferation, and differentiation of osteoblasts (MC3T3-E1) and fibroblasts (L929); additionally, the effect on apoptosis and matrix metalloproteinases (MMPs) expression in these cells was evaluated. Cell proliferation assay, alkaline phosphatase (ALP) activity assay, Western blotting, and quantitative real-time polymerase chain reaction were performed to determine the effects. The 10-MDP calcium salts (within a concentration of 0.5 mg/mL) showed no cytotoxicity and did not seem to influence the apoptosis, mitochondrial membrane potential, and reactive oxygen species (ROS) levels in the cells. However, they had an inhibitory effect on the secretion of MMP2 and MMP9 in the osteoblasts and fibroblasts. The ALP activity assay and Alizarin Red staining did not reveal any significant effects of the 10-MDP calcium salts on osteoblast differentiation. These results suggest that applying 10-MDP-containing adhesives to subgingival fillings may be safe and beneficial for the periodontal tissues. [Display omitted] • 10-MDP calcium salts directed contact the periodontium in the case of subgingival resin filling. • 10-MDP calcium salts have inhibitory effect on the secretion of MMP2 and MMP9 in the osteoblasts and fibroblasts. • 10-MDP calcium salts showed no cytotoxicity and influences on the mitochondria in osteoblasts and fibroblasts. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01095641
Volume :
40
Issue :
9
Database :
Academic Search Index
Journal :
Dental Materials
Publication Type :
Academic Journal
Accession number :
179028803
Full Text :
https://doi.org/10.1016/j.dental.2024.06.015